5 Expression Systems
>95% Purity Guaranteed
µg to kg Scale
GMP Manufacturing
Sequence & Activity QC
Service Overview
Comprehensive Protein Expression for Every Research Need
ICDMO's recombinant protein expression services deliver high-quality proteins for structural biology, drug target validation, antibody development, enzyme engineering, and biopharmaceutical manufacturing. Five orthogonal expression platforms operate in parallel, enabling our scientists to match the optimal system to each target based on size, glycosylation, disulfide bonds, and required quantity.
Our pipeline integrates codon optimization, vector engineering, fermentation or cell culture scale-up, and multi-step chromatography into a seamless one-stop service. Every batch undergoes rigorous QC before delivery.
1,000+
Proteins Expressed
>95%
Typical Purity
19+
Years Experience
Expression Systems
Five Platforms, One Partner
Each system is maintained by dedicated protein scientists with platform-specific expertise.
Key Advantages
Multi-System Expertise
Five expression platforms in-house. Our scientists match the optimal system to your target's requirements and application.
High Purity Guaranteed
Multi-step chromatography delivers >95% purity with <1.0 EU/µg endotoxin. SEC-HPLC purity profile included for every batch.
Flexible Scale
Microgram quantities for structural studies through kilogram-scale GMP manufacturing for clinical programs.
Comprehensive QC
SDS-PAGE, Western blot, SEC-HPLC, LC-MS, DLS, endotoxin, bioactivity assay, and full CoA documentation.
Rapid Feasibility
In-house bioinformatics analysis flags expression challenges before project start, minimizing timeline risk.
GMP Capability
cGMP production in ISO 7 cleanrooms with full batch records and regulatory documentation for IND programs.
What We Offer
Custom Protein Production
▸Enzymes, receptors, antibody fragments, growth factors, cytokines, structural proteins
▸Soluble, secreted, or inclusion body refolding depending on system and target
▸Membrane protein expression with detergent or nanodisc reconstitution
▸Multi-subunit complex co-expression using polycistronic vectors or co-infection
Protein Purification
▸Affinity: IMAC (His), GST, MBP, Strep-tag, antibody affinity chromatography
▸Ion exchange (IEX) and hydrophobic interaction (HIC) polishing steps
▸Size-exclusion chromatography (SEC) for aggregate removal and oligomeric profiling
▸TEV / thrombin protease tag removal and re-purification of tag-free product
Analytical & Quality Services
▸SDS-PAGE (reducing & non-reducing), Western blot for identity confirmation
▸SEC-HPLC purity profile; LC-MS / MALDI-TOF for molecular weight verification
▸DLS for hydrodynamic radius; LAL endotoxin assay (<1.0 EU/µg standard)
▸Bioactivity assay design: enzyme kinetics, ELISA, SPR, cell-based functional assays
Service Workflow
1
Sequence Analysis & Strategy
Bioinformatics review: signal peptide, TM domains, disulfide bonds, glycosylation sites. System, vector, and tag strategy recommended.
2
Vector Construction
Codon-optimized gene cloned into proprietary vector. Tag position and protease cleavage site (TEV/thrombin) selected for downstream use.
3
Small-Scale Screening
Expression tested across temperatures, inducers, and media. Solubility and yield confirmed by SDS-PAGE and Western blot before scale-up.
4
Scale-Up Production
Optimal condition applied at production scale — shake flask, bioreactor, or spinner flask. Monitored throughout for yield and cell health.
5
Purification
Multi-step chromatography: affinity capture → IEX polishing → SEC finishing. Optional tag removal by protease digestion and re-purification.
6
QC & Delivery
SDS-PAGE, SEC-HPLC, LC-MS, endotoxin LAL. Shipped on dry ice with CoA, sequence trace, and full data package.
Service Process
1
Online Consultation
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2
Solution Matching
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3
Service Contract
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4
Project Execution
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5
Data Delivery
Specifications & Turnaround
Note: All services are for research use only unless specified as GMP. Contact us for IND-enabling or clinical manufacturing documentation.
Get a Custom Quote
Protein scientists respond within 24 hours with a feasibility assessment and detailed proposal.
Contact Us Online ConsultationStandard Deliverables
✓Purified protein (specified qty)
✓SDS-PAGE & Western blot
✓SEC-HPLC purity profile
✓LC-MS MW confirmation
✓Endotoxin test report
✓Certificate of Analysis (CoA)
Why ICDMO?
5 Expression Systems In-House
1,000+ Proteins Expressed
GMP Manufacturing Available
Full Analytical QC Package
19+ Years Experience